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                      首頁 > 技術(shù)文章 >CEM微波合成用于γ-Lactam的研究

                      CEM微波合成用于γ-Lactam的研究

                       更新時間:2012-06-29 點擊量:1952

                      INTRODUCTION

                      One challenge facing peptide synthesis is the -lactam formation of arginine (Figure 1). The formation of -lactam occurs when the internal nitrogen of the guanidine attacks the activated ester, irreversibly forming the lactam ring. This reaction is competitive with peptide-bond formation, greatly reducing the coupling efficiency of arginine.

                      Figure 1: γ-Lactam Formation

                      The goal of this study was to determine what effect microwave irradiation has on -lactam formation and how to optimize the microwave synthesis of arginine-containing peptides. As a test substrate, we selected the 1992 ABRF study peptide sequence. This sequence is known to suffer from Arg deletion due to -lactam formation.

                       
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